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Addgene

pLenti4/V5-DEST-P110alpha
(Plasmid #40230)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti4-V5/DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6949
  • Total vector size (bp) 10200
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P110 alpha
  • Alt name
    PIK3CA
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    3200
  • Entrez Gene
    PIK3CA
  • Promoter CMV
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    HA-tagged bovine P110 alpha construct was provided by Dr Lewis Cantley (Beth Israel Deaconess Medical Center).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti4/V5-DEST-P110alpha was a gift from Lynda Chin (Addgene plasmid # 40230 ; http://n2t.net/addgene:40230 ; RRID:Addgene_40230)
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