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Addgene

pLenti4/V5-DEST-P85(KS459del)
(Plasmid #40222)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40222 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti4-V5/DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6949
  • Total vector size (bp) 9200
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    P85(KS459delN)
  • Alt name
    PIK3R1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2175
  • Mutation
    amino acids 459-460 replaced with asparagine
  • Entrez Gene
    PIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Flag-tagged human P85 construct was provided by Dr Jayne Stommel (National Cancer Institute).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti4/V5-DEST-P85(KS459del) was a gift from Lynda Chin (Addgene plasmid # 40222 ; http://n2t.net/addgene:40222 ; RRID:Addgene_40222)
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