pCMV Myc rat SAPAP4
(Plasmid #40218)

• Depositing Labs: Yutaka Hata, Yoshimi Takai
• Publication: Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51.

Backbone

• Vector backbone: pCMV5 (modified)
• Modifications to backbone: pCMV5 was modified to include an N-terminal Myc tag
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SAPAP4
• Species: R. norvegicus (rat)
• Mutation: N834D
• Entrez Gene: Dlgap4 (a.k.a. DAP-4, Dap4, SAPAP4)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SmaI, XmaI, BamHI or XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R (5'-CCAGCTTGGTTCCCAATAGA-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry