pCIneoMyc rat SAPAP1
(Plasmid #40215)

• Depositing Labs: Yutaka Hata, Yoshimi Takai
• Publication: Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51.

Backbone

• Vector backbone: pCI-neo Myc
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Modifications to backbone: pCI-neo was modified to include an N-terminal Myc tag
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SAPAP1
• Species: R. norvegicus (rat)
• Entrez Gene: Dlgap1 (a.k.a. DAP-1, GKAP/SAPAP1, Gkap, SAPAP1, rGKAP)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that after the stop codon of SAPAP1 and before the NotI site Addgene's sequencing results identified an untranslated portion of Cnksr2 (amino acids 850-1028 when compared GenBank ID NP_001106837.1)