pClneoMyc human ERBIN
(Plasmid #40214)

• Depositing Lab: Yutaka Hata
• Publication: Ohno et al Oncogene. 2002 Oct 10;21(46):7042-9.

Backbone

• Vector backbone: pCI-neo (modified)
• Backbone manufacturer: Promega
• Modifications to backbone: pCI-neo vector was modified to include N-terminal myc tag
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERBIN
• Species: H. sapiens (human)
• Mutation: L143S, S274L and P338L mutation in ERBIN compared to NCBI reference NP_001006600.1
• Entrez Gene: ERBIN (a.k.a. ERBB2IP, HEL-S-78, LAP2)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The Addgene verified sequence identified the mutations L143S, S274L, and P338L in ERBIN compared to NCBI reference NP_001006600.1. The functional effects of these mutations are unknown, however the plasmid functions as described in the associated publication.