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Addgene

pClneoMyc human ERBIN
(Plasmid #40214)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40214 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCI-neo (modified)
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    pCI-neo vector was modified to include N-terminal myc tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERBIN
  • Species
    H. sapiens (human)
  • Mutation
    L143S, S274L and P338L mutation in ERBIN compared to NCBI reference NP_001006600.1
  • Entrez Gene
    ERBIN (a.k.a. ERBB2IP, HEL-S-78, LAP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The Addgene verified sequence identified the mutations L143S, S274L, and P338L in ERBIN compared to NCBI reference NP_001006600.1. The functional effects of these mutations are unknown, however the plasmid functions as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoMyc human ERBIN was a gift from Yutaka Hata (Addgene plasmid # 40214 ; http://n2t.net/addgene:40214 ; RRID:Addgene_40214)
  • For your References section:

    Localization of p0071-interacting proteins, plakophilin-related armadillo-repeat protein-interacting protein (PAPIN) and ERBIN, in epithelial cells. Ohno H, Hirabayashi S, Iizuka T, Ohnishi H, Fujita T, Hata Y. Oncogene. 2002 Oct 10;21(46):7042-9. 10.1038/sj.onc.1205852 PubMed 12370826