pClneoMyc rat S-SCAM alpha
(Plasmid #40213)

• Depositing Labs: Yutaka Hata, Yoshimi Takai
• Publication: Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-10.

Backbone

• Vector backbone: pCI-neo (modified)
• Backbone manufacturer: Promega
• Modifications to backbone: pCI-neo vector was modified to include N-terminal myc tag
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S-SCAM
• Alt name: synaptic scaffolding molecule
• Species: R. norvegicus (rat)
• GenBank ID: AF034863
• Entrez Gene: Magi2 (a.k.a. AIP-1, Acvrinp1, Acvrip1, S-SCAM)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry