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Addgene

pcDNA6 MCV LTco
(Plasmid #40200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6.V5.HisB
  • Backbone manufacturer
    Invirtogen
  • Backbone size w/o insert (bp) 4985
  • Total vector size (bp) 7549
  • Modifications to backbone
    pcDNA6.V5.HisB vector was modified with NheI and PmeI restriction digestion enzymes to cut out MCS, V5 and His tag sites. New multiple cloning sites using annealed primer pair were ligated back into the vector. The newly generated cloning site comprises: NheI, KpnI, EcoRV, XhoI and SacII. Codon optimized Large T was cloned between EcoRV and XhoI restriction sites.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MCPyV LT codon optimized
  • Alt name
    MCV LT
  • Alt name
    MCV large T antigen
  • Alt name
    Merkel Cell Polyomavirus large T antigen
  • Species
    Virus
  • Insert Size (bp)
    2564
  • GenBank ID
    AEM01097.1
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA6 MCV LTco was a gift from Patrick Moore (Addgene plasmid # 40200 ; http://n2t.net/addgene:40200 ; RRID:Addgene_40200)

  • For your References section:

    Human Merkel cell polyomavirus small T antigen is an oncoprotein targeting the 4E-BP1 translation regulator. Shuda M, Kwun HJ, Feng H, Chang Y, Moore PS. J Clin Invest. 2011 Sep;121(9):3623-34. doi: 10.1172/JCI46323. Epub 2011 Aug 15. 10.1172/JCI46323 PubMed 21841310
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