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Addgene

pEQ111m
(Plasmid #40190)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSCC3
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3200
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    JC4588 lambda+
  • Growth instructions
    Induce expression by transfer to 37˚C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EcoRI endonuclease & EcoRI methylase
  • Species
    E. coli
  • Insert Size (bp)
    2210
  • Mutation
    changed Glutamic Acid 111 to Glutamine
  • GenBank ID
    J01675.1
  • Promoter Lambda PL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer NNNNNN
  • 3′ sequencing primer NNNNNN
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSCC3 plasmid derivation is described in King, K. et al. (1989) J.Biol.Chem. 264, 11807-11815.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEQ111m was a gift from Paul Modrich (Addgene plasmid # 40190 ; http://n2t.net/addgene:40190 ; RRID:Addgene_40190)

  • For your References section:

    The negative charge of Glu-111 is required to activate the cleavage center of EcoRI endonuclease. Wright DJ, King K, Modrich P. J Biol Chem. 1989 Jul 15;264(20):11816-21. PubMed 2745418
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