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Addgene

pLentiRhoA2G
(Plasmid #40179)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40179 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti pRRL-SV40(puro)_CMV(mcs)
  • Backbone manufacturer
    unknown
  • Backbone size w/o insert (bp) 7711
  • Total vector size (bp) 10316
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RhoA Biosensor
  • Species
    Synthetic
  • Insert Size (bp)
    2604
  • Promoter CMV
  • Tag / Fusion Protein
    • His-Myc (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CGACTCTAGAGGATCCGGAGATATACCATGG
  • 3′ sequencing primer CTAGACTGCAGGATCCTGGTGATGGTGATGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiRhoA2G was a gift from Olivier Pertz (Addgene plasmid # 40179 ; http://n2t.net/addgene:40179 ; RRID:Addgene_40179)

  • For your References section:

    A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space. Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O. Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135. 10.1126/scisignal.2004135 PubMed 23882122
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