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Addgene

pLentiEKAR2G2
(Plasmid #40178)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40178 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti pRRL-SV40(puro)_CMV(mcs)
  • Backbone manufacturer
    unknown
  • Backbone size w/o insert (bp) 7711
  • Total vector size (bp) 9935
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Erk activity reporter
  • Species
    Synthetic
  • Insert Size (bp)
    2224
  • Promoter CMV
  • Tag / Fusion Protein
    • His-Myc (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CGACTCTAGAGGATCCGGAGATATACCATGG
  • 3′ sequencing primer CTAGACTGCAGGATCCTGGTGATGGTGATGG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Karel Svoboda, Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A genetically encoded fluorescent sensor of ERK activity. 2008. PNAS 205(49)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLentiEKAR2G2 was a gift from Olivier Pertz (Addgene plasmid # 40178 ; http://n2t.net/addgene:40178 ; RRID:Addgene_40178)

  • For your References section:

    A Versatile Toolkit to Produce Sensitive FRET Biosensors to Visualize Signaling in Time and Space. Fritz RD, Letzelter M, Reimann A, Martin K, Fusco L, Ritsma L, Ponsioen B, Fluri E, Schulte-Merker S, van Rheenen J, Pertz O. Sci Signal. 2013 Jul 23;6(285):rs12. doi: 10.1126/scisignal.2004135. 10.1126/scisignal.2004135 PubMed 23882122
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