pGL4.20-HIF1A prom
(Plasmid #40173)

• Depositing Lab: Alex Minella
• Publication: Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95. Epub 2011 Jul 11.

Backbone

• Vector backbone: pGL4.20
• Backbone manufacturer: Promega
• Modifications to backbone: addition of HIF1A (human) promoter fragment
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HIF1A promoter fragment
• Species: H. sapiens (human)
• Insert Size (bp): 2208
• Entrez Gene: HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (not destroyed)
• 3′ cloning site: Hind3 (not destroyed)
• 5′ sequencing primer: pGL4 sequencing oligo
• 3′ sequencing primer: internal oligo

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry