pUC19-HRP-C
(Plasmid #40163)

• Depositing Lab: Paul Ortiz de Montellano
• Publication: Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1):61-72.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2700
• Total vector size (bp): 3700
• Vector type: coli cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Horseradish peroxidase
• Alt name: HRP
• Species: Synthetic; horseradish
• Insert Size (bp): 966
• Tag / Fusion Protein:
  • Six histidine (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• Supplemental Documents:
  • pUC19 HRP-C partial sequence and Asp-N endopeptidase sites

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Synthetic gene with codons optimized for mammalian expression. See original reference: Hartmann, C, and Ortiz de Montellano, P, "Baculovirus expression and characterization of catalytically active horseradish peroxidase" (1992) Arch Biochem Biophys, 297, 61-72.