pFM115
(Plasmid
#40161)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40161 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-6p-1
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Backbone manufacturerGE
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepar-3C
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Alt namepar-3
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SpeciesC. elegans (nematode)
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MutationC terminal portion of protein (aa850-1286)
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GenBank IDNC_003281.9
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Entrez Genepar-3 (a.k.a. CELE_F54E7.3)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pGEX5
- 3′ sequencing primer pGEX3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alternate plasmid name: pGEX6p1-par-3C
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFM115 was a gift from Geraldine Seydoux (Addgene plasmid # 40161 ; http://n2t.net/addgene:40161 ; RRID:Addgene_40161) -
For your References section:
Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes. Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G. Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. 10.1038/ncb2354 PubMed 21983565