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Addgene

pFM113
(Plasmid #40159)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40159 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6p-1
  • Backbone manufacturer
    GE
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    par-3N
  • Alt name
    par-3
  • Species
    C. elegans (nematode)
  • Mutation
    N terminal portion of protein (aa1-364)
  • GenBank ID
    NC_003281.9
  • Entrez Gene
    par-3 (a.k.a. CELE_F54E7.3)
  • Entrez Gene
    par-3 (a.k.a. CELE_F54E7.3)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternate plasmid name: pGEX6p1-par-3N

Note: During the Addgene quality control process, it was determined that this construct originally encoded a par-3 fragment containing amino acid residues 1 through 408, but a premature stop codon limits the protein to amino acid residue 364.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFM113 was a gift from Geraldine Seydoux (Addgene plasmid # 40159 ; http://n2t.net/addgene:40159 ; RRID:Addgene_40159)

  • For your References section:

    Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes. Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G. Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. 10.1038/ncb2354 PubMed 21983565
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