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pFM101
(Plasmid #40147)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6p-1
  • Backbone manufacturer
    GE
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gfp::par-2 wild-type RNAi resistant
  • Alt name
    par-2 wild-type RNAi resisitant
  • Alt name
    par-2
  • Species
    C. elegans (nematode)
  • Mutation
    Base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene.
  • GenBank ID
    NC_003281.9
  • Entrez Gene
    par-2 (a.k.a. CELE_F58B6.3)
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX5
  • 3′ sequencing primer pGEX3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternate plasmid name: pGEX6p1-gfp::par-2 wild-type

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFM101 was a gift from Geraldine Seydoux (Addgene plasmid # 40147 ; http://n2t.net/addgene:40147 ; RRID:Addgene_40147)

  • For your References section:

    Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes. Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G. Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. 10.1038/ncb2354 PubMed 21983565
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