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Addgene

pFM039
(Plasmid #40142)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pID3.01
  • Backbone manufacturer
    Addgene plasmid 22720
  • Backbone size w/o insert (bp) 18033
  • Vector type
    Worm Expression ; Gateway Destination vector
  • Selectable markers
    unc-119 rescuing fragment

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    par-2 RNAi resistant (R183-5A + S241A)
  • Alt name
    par-2
  • Species
    C. elegans (nematode)
  • Mutation
    R183-5A + S241A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene.
  • GenBank ID
    NC_003281.9
  • Entrez Gene
    par-2 (a.k.a. CELE_F58B6.3)
  • Promoter pie-1
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternate plasmid name: pID3.01-par-2RR R183-5A + S241A

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFM039 was a gift from Geraldine Seydoux (Addgene plasmid # 40142 ; http://n2t.net/addgene:40142 ; RRID:Addgene_40142)

  • For your References section:

    Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes. Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G. Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. 10.1038/ncb2354 PubMed 21983565
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