PRM-GFP
(Plasmid #40127)

• Depositing Lab: Michel Maharbiz
• Publication: Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Backbone

• Vector backbone: p15A
• Backbone size w/o insert (bp): 2097
• Total vector size (bp): 2965
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CI inducible sfGFP
• Species: E. Coli
• GenBank ID: JX155239
• Promoter: pRM
• Tag / Fusion Protein:
  • LVA ssrA tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI, BglII (not destroyed)
• 3′ cloning site: BamHI, XhoI (not destroyed)
• 5′ sequencing primer: GFP-F
• 3′ sequencing primer: CCCGAAGGTGAGCCAGTGTGACTCTAG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry