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Addgene

PLlacO-1-CImf
(Plasmid #40126)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40126 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Backbone size w/o insert (bp) 4147
  • Total vector size (bp) 4282
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Lac inducible lambda repressor
  • Species
    E. Coli
  • Insert Size (bp)
    833
  • GenBank ID
    JX155232
  • Tag / Fusion Protein
    • mf-lon ssrA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, BglII (not destroyed)
  • 3′ cloning site BamHI, XhoI (not destroyed)
  • 5′ sequencing primer CGTAGAGGATCGAGAATTGTGAGCG
  • 3′ sequencing primer CCCGAAGGTGAGCCAGTGTGACTCTAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PLlacO-1-CImf was a gift from Michel Maharbiz (Addgene plasmid # 40126 ; http://n2t.net/addgene:40126 ; RRID:Addgene_40126)

  • For your References section:

    A genetic bistable switch utilizing nonlinear protein degradation. Huang DC, Holtz WJ, Maharbiz MM. J Biol Eng. 2012 Jul 9;6(1):9. 10.1186/1754-1611-6-9 PubMed 22776405
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