pLenti6/V5-DEST-RINT1
(Plasmid
#40124)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6-V5/DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7732
- Total vector size (bp) 10108
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRINT1
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Alt nameRAD50 interactor 1
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SpeciesH. sapiens (human)
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Insert Size (bp)2376
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Entrez GeneRINT1 (a.k.a. ILFS3, RINT-1)
- Promoter CMV
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Tag
/ Fusion Protein
- V5 epitope (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer V5 reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe entry clone used to create this plasmid was obatined from the human ORFeome (v5.1) collection made available by the Center for Cancer Systems Biology at the Dana-Farber Cancer Institute in Boston, MA.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6/V5-DEST-RINT1 was a gift from Lynda Chin (Addgene plasmid # 40124 ; http://n2t.net/addgene:40124 ; RRID:Addgene_40124) -
For your References section:
Integrative functional genomics identifies RINT1 as a novel GBM oncogene. Quayle SN, Chheda MG, Shukla SA, Wiedemeyer R, Tamayo P, Dewan RW, Zhuang L, Huang-Hobbs E, Haidar S, Xiao Y, Ligon KL, Hahn WC, Chin L. Neuro Oncol. 2012 Nov;14(11):1325-31. doi: 10.1093/neuonc/nos246. Epub 2012 Oct 16. 10.1093/neuonc/nos246 PubMed 23074196
Map uploaded by the depositor.