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Addgene

pEG795
(Plasmid #40122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR P2RP3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4773
  • Vector type
    Gateway Donor vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mex-5 (ATG through 648 bp of 3'UTR) (RNAi resistant exon 2)
  • Alt name
    Mex-5
  • Species
    C. elegans (nematode)
  • Mutation
    Exon 2 recoded to be RNAi-resistant
  • GenBank ID
    NM_070165.4 NP_502566.1
  • Entrez Gene
    mex-5 (a.k.a. CELE_W02A2.7)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene.

Alternate plasmid name: pDONR P2RP3+MEX-5 genomic(RNAI res exon 2) + 3’UTR

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEG795 was a gift from Geraldine Seydoux (Addgene plasmid # 40122 ; http://n2t.net/addgene:40122 ; RRID:Addgene_40122)

  • For your References section:

    Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle. Griffin EE, Odde DJ, Seydoux G. Cell. 2011 Sep 16;146(6):955-68. 10.1016/j.cell.2011.08.012 PubMed 21925318
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