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Addgene

SW5
(Plasmid #40108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psc101
  • Backbone size w/o insert (bp) 3460
  • Total vector size (bp) 7582
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Bistable switch using dynamic degradation
  • Species
    E. Coli
  • GenBank ID
    JX155251

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI, BglII (not destroyed)
  • 3′ cloning site BamHI, XhoI (not destroyed)
  • 5′ sequencing primer GATCAGATCATGATCCCCTGCGCCATCAGATCC
  • 3′ sequencing primer CCCGAAGGTGAGCCAGTGTGACTCTAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SW5 was a gift from Michel Maharbiz (Addgene plasmid # 40108 ; http://n2t.net/addgene:40108 ; RRID:Addgene_40108)

  • For your References section:

    A genetic bistable switch utilizing nonlinear protein degradation. Huang DC, Holtz WJ, Maharbiz MM. J Biol Eng. 2012 Jul 9;6(1):9. 10.1186/1754-1611-6-9 PubMed 22776405
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