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Addgene

pEG495
(Plasmid #40089)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40089 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMal c2X (GW)
  • Backbone manufacturer
    New England Biolabs (Modified)
  • Backbone size w/o insert (bp) 6700
  • Total vector size (bp) 8100
  • Modifications to backbone
    Gateway acceptor sites added
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mex-5 (S404A, S458A)
  • Alt name
    Mex-5
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1407
  • Mutation
    S404A, S458A
  • GenBank ID
    NM_070165.4 NP_502566.1
  • Entrez Gene
    mex-5 (a.k.a. CELE_W02A2.7)
  • Promoter P-lac
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S404A, S458A)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEG495 was a gift from Geraldine Seydoux (Addgene plasmid # 40089 ; http://n2t.net/addgene:40089 ; RRID:Addgene_40089)

  • For your References section:

    Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle. Griffin EE, Odde DJ, Seydoux G. Cell. 2011 Sep 16;146(6):955-68. 10.1016/j.cell.2011.08.012 PubMed 21925318
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