Skip to main content
Addgene

pEG412
(Plasmid #40087)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pIC26
  • Backbone manufacturer
    Cheeseman lab
  • Backbone size (bp) 16397
  • Modifications to backbone
    GFP replaced with Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted)
  • Vector type
    Worm Expression
  • Promoter pie-1
  • Selectable markers
    unc-119
  • Tags / Fusion Proteins
    • Dendra2 PAFP (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene of interest should be cloned into the SpeI restriction site to generate Dendra2 FPAP2 N-terminal fusion proteins.

Alternate vector name: pIC26 + Dendra

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEG412 was a gift from Geraldine Seydoux (Addgene plasmid # 40087 ; http://n2t.net/addgene:40087 ; RRID:Addgene_40087)

  • For your References section:

    Regulation of the MEX-5 gradient by a spatially segregated kinase/phosphatase cycle. Griffin EE, Odde DJ, Seydoux G. Cell. 2011 Sep 16;146(6):955-68. 10.1016/j.cell.2011.08.012 PubMed 21925318
Related items:
Commonly requested with: