pLMB51
(Plasmid
#40083)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40083 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRP4
- Total vector size (bp) 13893
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namegusA
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SpeciesE. coli
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Insert Size (bp)1812
Gene/Insert 2
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Gene/Insert nametauR
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SpeciesSinorhizobium meliloti
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Insert Size (bp)1473
Gene/Insert 3
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Gene/Insert nametau promoter
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SpeciesSinorhizobium meliloti
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Insert Size (bp)157
Resource Information
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Addgene Notes
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GenBank accession number JQ895026
Multiple-cloning site (MCS) of pLMB51: GATTACCTCA GTCTAGAGCT CGAGAAGCTT GGATCCATGG TACCCTACA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLMB51 was a gift from Philip Poole (Addgene plasmid # 40083 ; http://n2t.net/addgene:40083 ; RRID:Addgene_40083) -
For your References section:
Regulatable vectors for environmental gene expression in alphaproteobacteria. Tett AJ, Rudder SJ, Bourdes A, Karunakaran R, Poole PS. Appl Environ Microbiol. 2012 Oct;78(19):7137-40. Epub 2012 Jul 20. 10.1128/AEM.01188-12 PubMed 22820336