pFM032
(Plasmid
#40076)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIC26
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Backbone manufacturerCheeseman lab
- Backbone size (bp) 16397
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Modifications to backboneGFP replaced with Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted)
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Vector typeWorm Expression
- Promoter pie-1
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Selectable markersunc-119
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Tags
/ Fusion Proteins
- Dendra2 PAFP (N terminal on backbone)
- TEV protease cleavage site (N terminal on backbone)
- S-peptide (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alternate plasmid name: pIC26 + wormanized Dendra:speI
Gene of interest should be cloned into the SpeI restriction site to generate Dendra2 FPAP2 N-terminal fusion proteins.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFM032 was a gift from Geraldine Seydoux (Addgene plasmid # 40076 ; http://n2t.net/addgene:40076 ; RRID:Addgene_40076) -
For your References section:
Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes. Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G. Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. 10.1038/ncb2354 PubMed 21983565