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pGEX4T-1 hSlp4-a I18A
(Plasmid #40066)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T-1
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 6900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Expression in BL21 with IPTG
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hSlp4-a I18A
  • Alt name
    Synaptotagmin-like protein 4-a
  • Alt name
    Slp4-a I18A
  • Alt name
    Granuphilin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2025
  • Mutation
    Isoleucine 18 to Alanine
  • GenBank ID
    NM_080737
  • Entrez Gene
    SYTL4 (a.k.a. SLP4)
  • Promoter Tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T-1 hSlp4-a I18A was a gift from Fernando Martin-Belmonte (Addgene plasmid # 40066 ; http://n2t.net/addgene:40066 ; RRID:Addgene_40066)

  • For your References section:

    Synaptotagmin-like proteins control the formation of a single apical membrane domain in epithelial cells. Galvez-Santisteban M, Rodriguez-Fraticelli AE, Bryant DM, Vergarajauregui S, Yasuda T, Banon-Rodriguez I, Bernascone I, Datta A, Spivak N, Young K, Slim CL, Brakeman PR, Fukuda M, Mostov KE, Martin-Belmonte F. Nat Cell Biol. 2012 Jul 22. doi: 10.1038/ncb2541. 10.1038/ncb2541 PubMed 22820376
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