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Addgene

pDEST15 hSlp4-a
(Plasmid #40046)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40046 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDEST15
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 9000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 strain for expression (w/IPTG)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hSlp4-a
  • Alt name
    Synaptotagmin-like protein 4-a
  • Alt name
    Slp4-a WT
  • Alt name
    Granuphilin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2025
  • GenBank ID
    NM_080737
  • Entrez Gene
    SYTL4 (a.k.a. SLP4)
  • Promoter T7
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST15 hSlp4-a was a gift from Fernando Martin-Belmonte (Addgene plasmid # 40046 ; http://n2t.net/addgene:40046 ; RRID:Addgene_40046)
  • For your References section:

    Synaptotagmin-like proteins control the formation of a single apical membrane domain in epithelial cells. Galvez-Santisteban M, Rodriguez-Fraticelli AE, Bryant DM, Vergarajauregui S, Yasuda T, Banon-Rodriguez I, Bernascone I, Datta A, Spivak N, Young K, Slim CL, Brakeman PR, Fukuda M, Mostov KE, Martin-Belmonte F. Nat Cell Biol. 2012 Jul 22. doi: 10.1038/ncb2541. 10.1038/ncb2541 PubMed 22820376