pRc/CMV HA TrkB
(Plasmid #39978)

• Depositing Lab: Yves-Alain Barde
• Publication: Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Backbone

• Vector backbone: pRC/CMV AC7 vector, a derivative of the pRC/CMV vector
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TrkB
• Species: R. norvegicus (rat)
• Insert Size (bp): 2500
• Mutation: endogenous signal sequence was replaced with BM40 and HA tag
• GenBank ID: NM_012731.2
• Entrez Gene: Ntrk2 (a.k.a. RATTRKB1, TRKB1, Tkrb, trk-B, trkB)
• Promoter: CMV
• Tags / Fusion Proteins:
  • BM40 signal sequence (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (PmeI before HindIII) (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide

Please note that the endogenous signal sequence of TrkB was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55291.1 or NM_012731.2, the TrkB protein sequence starts at amino acid #32