pRc/CMV HA TrkA
(Plasmid #39977)

• Depositing Lab: Yves-Alain Barde
• Publication: Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Backbone

• Vector backbone: pRC/CMV AC7 vector, a derivative of the pRC/CMV vector
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TrkA
• Alt name: trk
• Alt name: Ntrk1
• Species: R. norvegicus (rat)
• Insert Size (bp): 2500
• Mutation: endogenous signal sequence was replaced with BM40 and HA tag
• GenBank ID: NM_021589.1
• Entrez Gene: Ntrk1 (a.k.a. Trk)
• Promoter: CMV
• Tags / Fusion Proteins:
  • BM40 signal sequence (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (pmeI added) (unknown if destroyed)
• 3′ cloning site: Not I (internal NotI site was silently mutated) (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide

Please note that the endogenous signal sequence of TrkA was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to GenBank ID NP_067600.1, the TrkA protein sequence starts at amino acid #30