Skip to main content
Addgene

pGEX-6P-Cdh1wt (570)
(Plasmid #39877)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX-6T
  • Backbone manufacturer
    Amersham
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FZR1
  • Alt name
    CDH1
  • Alt name
    fizzy/cell division cycle 20 related 1 (Drosophila)
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_016263
  • Entrez Gene
    FZR1 (a.k.a. CDC20C, CDH1, DEE109, FZR, FZR2, HCDH, HCDH1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GST forward
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this construct has an additional mutation in Cdh1 - R52G.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-6P-Cdh1wt (570) was a gift from Jonathon Pines (Addgene plasmid # 39877 ; http://n2t.net/addgene:39877 ; RRID:Addgene_39877)
Related items:
Commonly requested with: