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pUHD-YFP-Plk1(T210D) (823)
(Plasmid #39875)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39875 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUHD 10-3
  • Backbone manufacturer
    Manfred Gossen
  • Total vector size (bp) 3146
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Plk1(T210D)
  • Species
    H. sapiens (human)
  • Mutation
    T210D = Constitutively Active
  • Entrez Gene
    PLK1 (a.k.a. PLK, STPK13)
  • Tag / Fusion Protein
    • YFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI/XbaI (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer LNCX primer
  • 3′ sequencing primer sv40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this construct has an additional mutation in Plk1 - P23S.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUHD-YFP-Plk1(T210D) (823) was a gift from Jonathon Pines (Addgene plasmid # 39875)

  • For your References section:

    Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. van de Weerdt BC, van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH. Mol Cell Biol. 2005 Mar;25(5):2031-44. 10.1128/MCB.25.5.2031-2044.2005 PubMed 15713655
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