pcDNA3-cdc2-AF (718)
(Plasmid #39872)

• Depositing Lab: Jonathon Pines
• Publication: Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cdc2
• Species: H. sapiens (human)
• Mutation: AF = T14A, Y15F
• Entrez Gene: CDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Phosphorylation site mutant of cdc2/Cdk1 that cannot be down-regulated by Wee1. Please note that this construct may also have a few mismatches from the available sequence for the CDK1 UTR. These changes do not affect plasmid function.