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pcDNA3/CyclinB1-GFP (28)
(Plasmid #39871)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39871 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CyclinB1
  • Species
    H. sapiens (human)
  • Mutation
    V5A
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer GFPfusionREV_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3/CyclinB1-GFP (28) was a gift from Jonathon Pines (Addgene plasmid # 39871 ; http://n2t.net/addgene:39871 ; RRID:Addgene_39871)

  • For your References section:

    Cyclin A- and cyclin E-Cdk complexes shuttle between the nucleus and the cytoplasm. Jackman M, Kubota Y, den Elzen N, Hagting A, Pines J. Mol Biol Cell. 2002 Mar;13(3):1030-45. 10.1091/mbc.01-07-0361 PubMed 11907280
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