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Addgene

pDisplay-mSA-EGFP-TM
(Plasmid #39863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDisplay
  • Backbone manufacturer
    Life Technology
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    monomeric streptavidin, EGFP, TM helix
  • Species
    Synthetic
  • GenBank ID
    AFA52653.1
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • FLAG (C terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-mSA-EGFP-TM was a gift from Sheldon Park (Addgene plasmid # 39863 ; http://n2t.net/addgene:39863 ; RRID:Addgene_39863)

  • For your References section:

    Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection. Lim KH, Huang H, Pralle A, Park S. Biotechnol Bioeng. 2013 Jan;110(1):57-67. doi: 10.1002/bit.24605. Epub 2012 Aug 8. 10.1002/bit.24605 PubMed 22806584
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