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pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)
(Plasmid #39858)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39858 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5-FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    Hygromycin resistance gene has been replaced with neomycin resistance gene.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    C11orf51
  • Species
    H. sapiens (human)
  • Entrez Gene
    ANAPC15 (a.k.a. APC15, C11orf51, HSPC020)
  • Tag / Fusion Protein
    • IRES2-mRuby (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI/PspOMI (destroyed during cloning)
  • 5′ sequencing primer LNCX primer
  • 3′ sequencing primer BGH_rev_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Can be used to rescue siC11orf51-4 from Pines lab.

C11orf51 and mRuby are separated by IRES2 and therefore are not fused.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763) was a gift from Jonathon Pines (Addgene plasmid # 39858 ; http://n2t.net/addgene:39858 ; RRID:Addgene_39858)

  • For your References section:

    APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment. Mansfeld J, Collin P, Collins MO, Choudhary JS, Pines J. Nat Cell Biol. 2011 Sep 18;13(10):1234-43. doi: 10.1038/ncb2347. 10.1038/ncb2347 PubMed 21926987
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