pcDNA3-myc-ΔD-box-CycA-GFP (525)
(Plasmid #39856)

• Depositing Lab: Jonathon Pines
• Publication: den Elzen et al J Cell Biol. 2001 Apr 2;153(1):121-36.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cyclin A2
• Species: H. sapiens (human)
• Mutation: L2V; Δ destruction box = deletion of aa47-55
• Entrez Gene: CCNA2 (a.k.a. CCN1, CCNA)
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NheI/XbaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry