pSG5-Bcr
(Plasmid
#39855)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39855 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 8400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBCR
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SpeciesH. sapiens (human)
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Insert Size (bp)4300
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Mutationwild type
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Entrez GeneBCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed by Leena Haataja.
BCR was inserted in a 4-way ligation of 1.6 kb EcoRI-SstI + 1.0 kb SstI-HindIII + 1.6 kb HindIII-BamHI fragments into pSG5 digested with EcoRI –BamHI. The construct contains no tag. The BCR cDNA 5'UT sequences were truncated to remove some of the GC-rich sequences. The construct includes the complete coding region, the natural stop codon and 3'UT sequences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-Bcr was a gift from Nora Heisterkamp (Addgene plasmid # 39855 ; http://n2t.net/addgene:39855 ; RRID:Addgene_39855)