pT7-Venus-Emi1 (914)
(Plasmid #39854)

• Depositing Lab: Jonathon Pines
• Publication: Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

Backbone

• Vector backbone: pT7-Venus-C1
• Modifications to backbone: T7 promoter was inserted downstream of the CMV promoter, and EYFP was replaced with Venus using AgeI and BsrGI. SacI and HindIII in MCS are no longer unique.
• Vector type: in vitro transcription
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Emi1
• Species: H. sapiens (human)
• Entrez Gene: FBXO5 (a.k.a. EMI1, FBX5, Fbxo31)
• Promoter: T7
• Tag / Fusion Protein:
  • Venus (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI/EcoRI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry