CycA-Venus-Flag (1305)
(Plasmid #39853)

• Depositing Lab: Jonathon Pines
• Publication: Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Pines lab
• Modifications to backbone: First, Venus-Flag was added to pcDNA5/FRT/TO between BstXI and XhoI. Second, an attR1-CmR-ccdB-attR2 GATEWAY cassette was inserted into at the AflII site.
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cyclin A
• Species: H. sapiens (human)
• Entrez Gene: CCNA2 (a.k.a. CCN1, CCNA)
• Tags / Fusion Proteins:
  • Venus (C terminal on backbone)
  • Flag (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: BGH_rev_primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry