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pCyclinB1(L45A,R42A)-mCherry (1963)
(Plasmid #39852)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39852 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CyclinB1
  • Species
    H. sapiens (human)
  • Mutation
    L45A and R42A
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV fwd
  • 3′ sequencing primer mCherry Rev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCyclinB1(L45A,R42A)-mCherry (1963) was a gift from Jonathon Pines (Addgene plasmid # 39852 ; http://n2t.net/addgene:39852 ; RRID:Addgene_39852)

  • For your References section:

    Activation of cyclin B1-Cdk1 synchronizes events in the nucleus and the cytoplasm at mitosis. Gavet O, Pines J. J Cell Biol. 2010 Apr 19;189(2):247-59. 10.1083/jcb.200909144 PubMed 20404109
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