pFAST-BAC-Cdc20wt (2193)
(Plasmid
#39850)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39850 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFAST-BAC B
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Backbone manufacturerInvitrogen
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Vector typeBaculovirus expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCdc20
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SpeciesH. sapiens (human)
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Entrez GeneCDC20 (a.k.a. CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer Polyhedrin (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFAST-BAC-Cdc20wt (2193) was a gift from Jonathon Pines (Addgene plasmid # 39850 ; http://n2t.net/addgene:39850 ; RRID:Addgene_39850) -
For your References section:
The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction. Nilsson J, Yekezare M, Minshull J, Pines J. Nat Cell Biol. 2008 Dec . 10(12):1411-20. 10.1038/ncb1799 PubMed 18997788