pVenus_Lys-less-Cdc20 (1620)
(Plasmid #39849)

• Depositing Lab: Jonathon Pines
• Publication: Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20.

Backbone

• Vector backbone: pVenus-C1
• Backbone manufacturer: Pines lab
• Modifications to backbone: EYFP in pEYFP-C1 was swapped for Venus using AgeI and BsrGI.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cdc20
• Species: H. sapiens (human)
• Mutation: Lys-less = all Lys converted to Arg
• Entrez Gene: CDC20 (a.k.a. CDC20A, OOMD14, OZEMA14, bA276H19.3, p55CDC)
• Tag / Fusion Protein:
  • Venus (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

RNAi resistant Cdc20 that cannot be ubiquitinated.