pEYFP-Pds1Δdestruction box(383)
(Plasmid #39848)

• Depositing Lab: Jonathon Pines
• Publication: Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37. Epub 2002 Jun 17.

Backbone

• Vector backbone: pEYFP-N1
• Backbone manufacturer: Clontech
• Total vector size (bp): 5307
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pds1
• Alt name: PTTG1
• Alt name: Securin
• Species: H. sapiens (human)
• Mutation: Δdestruction box = ΔR61-N68
• Entrez Gene: PTTG1 (a.k.a. EAP1, ECRAR, HPTTG, PTTG, TUTR1)
• Tag / Fusion Protein:
  • EYFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry