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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEYFP-C3
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePlk1
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SpeciesH. sapiens (human)
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Entrez GenePLK1 (a.k.a. PLK, STPK13)
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Tag
/ Fusion Protein
- EYFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEYFP-Plk1(499) was a gift from Jonathon Pines (Addgene plasmid # 39843 ; http://n2t.net/addgene:39843 ; RRID:Addgene_39843) -
For your References section:
Ordered proteolysis in anaphase inactivates Plk1 to contribute to proper mitotic exit in human cells. Lindon C, Pines J. J Cell Biol. 2004 Jan 19;164(2):233-41. 10.1083/jcb.200309035 PubMed 14734534