Torso cDNA Y918F in pGEM
(Plasmid
#39797)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-7Zf
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTorso
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Alt nametor
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SpeciesD. melanogaster (fly)
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MutationY918F
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Entrez Genetor (a.k.a. Dmel_CG1389, CG1389, Dmel\CG1389, TOR, TORSO, Tor, Torso, dTor, splc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer M13pUC-rev
- 3′ sequencing primer M13pUC-fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Perrimon Lab plasmid #215
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Torso cDNA Y918F in pGEM was a gift from Norbert Perrimon (Addgene plasmid # 39797 ; http://n2t.net/addgene:39797 ; RRID:Addgene_39797) -
For your References section:
Synergistic activities of multiple phosphotyrosine residues mediate full signaling from the Drosophila Torso receptor tyrosine kinase. Gayko U, Cleghon V, Copeland T, Morrison DK, Perrimon N. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):523-8. 10.1073/pnas.96.2.523 PubMed 9892666