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Addgene

Torso cDNA Y918F in pGEM
(Plasmid #39797)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39797 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEM-7Zf
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Torso
  • Alt name
    tor
  • Species
    D. melanogaster (fly)
  • Mutation
    Y918F
  • Entrez Gene
    tor (a.k.a. Dmel_CG1389, CG1389, Dmel\CG1389, TOR, TORSO, Tor, Torso, dTor, splc)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer M13pUC-rev
  • 3′ sequencing primer M13pUC-fwd
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Perrimon Lab plasmid #215

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Torso cDNA Y918F in pGEM was a gift from Norbert Perrimon (Addgene plasmid # 39797 ; http://n2t.net/addgene:39797 ; RRID:Addgene_39797)
  • For your References section:

    Synergistic activities of multiple phosphotyrosine residues mediate full signaling from the Drosophila Torso receptor tyrosine kinase. Gayko U, Cleghon V, Copeland T, Morrison DK, Perrimon N. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):523-8. 10.1073/pnas.96.2.523 PubMed 9892666