porcupine - Xenopus
(Plasmid
#39772)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39772 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBlueScript II KS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameporcupine
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SpeciesX. laevis (frog)
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Entrez Geneporcn (a.k.a. Xporc, xporc-d)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Perrimon Lab plasmid #126
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
porcupine - Xenopus was a gift from Norbert Perrimon (Addgene plasmid # 39772 ; http://n2t.net/addgene:39772 ; RRID:Addgene_39772) -
For your References section:
The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing. Kadowaki T, Wilder E, Klingensmith J, Zachary K, Perrimon N. Genes Dev. 1996 Dec 15;10(24):3116-28. 10.1101/gad.10.24.3116 PubMed 8985181