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D-raf R217L+S620A
(Plasmid #39765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSpeR-4
  • Backbone size w/o insert (bp) 7855
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    d-raf
  • Species
    D. melanogaster (fly)
  • Mutation
    R217L and S620A
  • Entrez Gene
    Raf (a.k.a. Dmel_CG2845, 11-29, C110, CG2845, D-RAF, D-Raf, D-raf, D-raf1, DRaf, DRaf1, Dmel\CG2845, Draf, Draf-1, Draf1, EG:BACH48C10.3, Phl, RAF, Raf/phl, Raf1, dRaf, draf, l(1)2Fe, l(1)G0475, l(1)ph, l(1)phl, l(1)pole hole, l(1)polehole, l(1)polehole/draf, l(1)raf, lincRNA.937, ph, ph1, phl, raf, raf-1, raf1, rafl)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Perrimon Lab plasmid #89

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D-raf R217L+S620A was a gift from Norbert Perrimon (Addgene plasmid # 39765 ; http://n2t.net/addgene:39765 ; RRID:Addgene_39765)

  • For your References section:

    Genetic and molecular analyses of mutations involved in Drosophila raf signal transduction. Lu X, Melnick MB, Hsu JC, Perrimon N. EMBO J. 1994 Jun 1;13(11):2592-9. PubMed 8013459
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