pEGFP-Akt-DD
(Plasmid #39536)

• Depositing Lab: Julian Downward
• Publication: Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Akt
• Species: M. musculus (mouse)
• Insert Size (bp): 1600
• Mutation: DD = T308D and S473D
• Entrez Gene: Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • EGFP (N terminal on backbone)
  • 6G linker (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: sv40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry