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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 39529 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepDONR221
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Backbone manufacturerLife Technologies (Invitrogen)
- Backbone size w/o insert (bp) 2500
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Vector typeGateway compatible cloning vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLRRK2
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SpeciesH. sapiens (human)
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Insert Size (bp)7584
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GenBank IDNM_198578.3
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Entrez GeneLRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13pUC-Fwd
- 3′ sequencing primer M13rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR221 LRRK2 wt was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 39529 ; http://n2t.net/addgene:39529 ; RRID:Addgene_39529)