pEGFP-ARAP3(R1155E)
(Plasmid
#39488)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 39488 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameARAP3(R1155E)
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SpeciesH. sapiens (human)
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MutationR1155E = Ras binding domain mutation
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Entrez GeneARAP3 (a.k.a. CENTD3, DRAG1)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer sv40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-ARAP3(R1155E) was a gift from Sonja Vermeren (Addgene plasmid # 39488 ; http://n2t.net/addgene:39488 ; RRID:Addgene_39488) -
For your References section:
ARAP3 is a PI3K- and rap-regulated GAP for RhoA. Krugmann S, Williams R, Stephens L, Hawkins PT. Curr Biol. 2004 Aug 10;14(15):1380-4. 10.1016/j.cub.2004.07.058 PubMed 15296756